Testicular Biopsy for Infertility: Procedure & Recovery


Testicular biopsy is an effective technique for diagnosing the cause of male infertility, cancer tissue, or sperm harvesting in men with azoospermia. In most cases, a biopsy is performed surgically, under local anesthesia, and by the hands of a specialist or a trained health professional. If you have more questions about the procedure, complications, and recovery period of testicular biopsy, continue reading.

What Is Testicular Biopsy?

A testicular biopsy involves removing a small piece of the testicle to check for sperm production. The process of producing sperm takes place in the seminiferous tubules in the testicles. In the seminiferous tubules, germ cells develop into mature sperms within 64 days and through 16 stages.

By examining the samples obtained from testicular biopsy, the doctor can check the presence or absence of germ cells and sperms as well as the development of the germ cells. If there is a healthy sperm, it can be extracted and used in IVF and other assisted reproductive techniques.

Testicular biopsy is one of the methods of analyzing fertility problems in men.

What Can I Expect from a Testicular Biopsy?

In a testicular biopsy, a small piece of one or both testicles is removed and examined by a urologist or fertility specialist. Usually, the biopsy technique is used to diagnose the cause of infertility in patients with normal FSH levels (Follicle-stimulating Hormone). In these patients, it is not possible to distinguish obstructive azoospermia from spermatogenesis defects or through a spermogram test.

If the testicular biopsy results are normal, the patient does not have testicular cancer, and the organs responsible for producing the sperm are functioning well. However, if the test results are abnormal, it shows that the patient has lumps, azoospermia, or orchitis and needs further treatments. 

Although testicular biopsy is a simple procedure, you have to expect some side effects, such as pain in the testes, possible bleeding, infection, swelling, discoloration of the scrotum, and discomfort while sleeping. 

Why Is Testicular Biopsy Done?

Testicular biopsy is used to diagnose sperm problems such as insufficiencies and diseases; also, it is helpful to retrieve sperm from the testicle to be used in IVF and other fertility treatment techniques such as IUI and ICSI.

Of course, it’s worth mentioning that not all azoospermic patients need a biopsy test. Doctors will not take the biopsy test if the azoospermia is due to the lack of seminal vesicles on both sides. Since sperm production is different in various parts of the testicle, a biopsy is done at different points. Today, four parts are considered for the testicle, and a testicular biopsy is done on each part.

After testicular biopsy for IVF, if healthy sperm is collected, the samples are frozen for later use. The sperms are frozen because, in some cases, these samples are the only sperm left for the patient, and the doctor may be unable to find mature sperms in subsequent biopsies.

The best candidates for testicular biopsy are those who have very few sperm in their semen

Testicular Biopsy for Azoospermia

Having no sperm in at least two semen samples is called azoospermia. This problem is caused when there is a blockage in ejaculation tracts or when the testes do not produce sperm. Although the only definitive way to diagnose azoospermia is the testicular biopsy, sometimes blood tests, clinical examinations, and genetic tests are used.

Azoospermia treatment
Read more: Azoospermia Treatments (Obstructive & Non-obstructive)


For men with azoospermia, testicular biopsy is done to harvest sperm from their testes to be used in ICSI or IVF. Of course, when the male partner doesn’t have the bilateral seminal vesicles, performing a biopsy is useless.

It is worth mentioning that in the past, testicular biopsy for azoospermia used to be done on a single part of the testicle. Still, several samples are received from different parts of the testicles because each part of the testicle produces a certain amount of sperm; maybe the doctor can find sperm in one specific part of the testicle.

Azoospermia Treatments

Best Candidates for Testicular Biopsy

The best candidates for testicular biopsy are those who have very few sperm in their semen analysis, and the following factors cause their sperm problem:

  • Congenital lack or blockage of the vas deferens;
  • Hormonal problems;
  • Radiation exposure, especially in patients with cancer;
  • Genetic factors and the formation of sperm-producing genes on the Y chromosome;
  • Infectious diseases such as mumps;
  • Some drugs, especially the ones used in cancer treatment, etc.


Preparation for a Testicular Biopsy

Before doing a testicular biopsy, the patient should take care of himself and inform the doctor about the following points:

  • Any kind of bleeding;
  • Having an allergy to any drug, including anesthetics; and
  • The medications he takes.


Also, the patient must shave the hair on the scrotum, around the penis, and groin the night before surgery.

Usually, a biopsy is done under local anesthesia. Still, if it has to be done under general anesthesia at the doctor's discretion, the patient should avoid eating and drinking before the surgery. 

What is Testicular Biopsy Procedure?

Testicular biopsy for IVF is done in four ways; percutaneous epididymal sperm aspiration (PESA), testicular sperm extraction (TESE), testicular sperm aspiration (TESA), and microsurgical epididymal sperm aspiration (MESA).

Testicular biopsy can be done in different ways.


In the following, all these methods are introduced in detail:

Percutaneous Epididymal Sperm Aspiration (PESA)

The PESA technique is used in cases where the patient has obstructive azoospermia; it means that although his testicles produce sperm, the sperm cannot enter the semen due to congenital disabilities, damage to the tubes that carry sperm, and infection. 

Before doing PESA, the doctor will usually advise you to shave your genital hair and attend the surgery center on an empty stomach in the morning. After sterilizing the room and administering local anesthesia, the sperms are extracted from the epididymis (inner tube of the testicle) with a syringe and insulin needle. Then, the sperm sample is delivered to the laboratory for further examination. 

If the test is positive, the person can be a candidate for microinjection; but if no sperm found in the testicular biopsy, the patient should be surgically treated. The doctors advise this method to reduce the costs and avoid testicular sampling.

ICSI in Iran
Read more: What is ICSI? + Difference between IVF and ICSI


Testicular Sperm Extraction (TESE)

In TESE, the patient should shave off his genital hair and attend the surgery center on an empty stomach in the morning. A caregiver is usually needed for TESE because this surgery is invasive. In this method, the surgery room is also sterilized, and local anesthesia is administered; however, it is possible to inject intravenous sedatives at the doctor's discretion and the patient's request. 

The appropriate drug should be injected into the spermatic cord (blood vessels connecting the testicles to the abdominal cavity) as well as the incision placed to anesthetize the patient.

After examining the testis's tissue under laboratory microscopes, if sperm is observed, the microinjection method can be used to treat the infertile man.

What to Do If No Sperm Found in TESE?

If no sperm are found in the testicular biopsy, the doctor has to repeat the biopsy to ensure that the patient has azoospermia. In such cases, the Micro TESE method (testicular biopsy with a microscope) is repeated to enlarge the target tissue and better detect the presence or absence of sperm.

Testicular Sperm Extraction (TESE) method

Testicular Sperm Aspiration (TESA)

In the TESA method, a less invasive procedure done under local anesthesia, a sample of testicular fluid and tissue is obtained with the help of a special needle. In fact, during the TESA operation, the sperms are gently pulled out just by making an incision in the scrotum’s skin. Then, the sperm needed for IVF or ICSI is retrieved from testicular fluid and tissue in the laboratory. This technique is also used for diagnosing azoospermia. 

Microsurgical Epididymal Sperm Aspiration (MESA)

The MESA method is used when the patient suffers from epididymis obstruction caused by vasectomy or congenital bilateral absence of the vas deferens. In this method, after carefully observing the epididymal tubes under the microscope, the doctor extracts healthy and motile sperm and uses them in IVF or ICSI. The doctor may also freeze some high-quality sperm for future use.

Is Testicular Biopsy Painful?

Typically, testicular biopsy is not as painful as done under general or local anesthesia. However, some patients who have undergone this procedure assert that they felt mild pain and pressure in their scrotum during the operation and a couple of days after, but the discomfort was eliminated very soon. 

Testicular Biopsy Side Effects

A testicular biopsy for IVF is a simple operation, and the person will not have severe bleeding or infection; of course, mild pain and swelling are perfectly normal. Some people are worried about the aggravation of fertility problems after the operation. We should note that this operation will not affect the erection or heighten infertility, so the patients should not be worried.

In case of observing the following problems after testicular biopsy, contact your doctor:

  • Severe pain;
  • Extreme swelling;
  • Fever above 38 degrees; and
  • Severe bleeding.


Testicular Biopsy Recovery Time

Although testicular biopsy is not painful, you must follow some postoperative instructions during recovery to minimize discomfort and complications. It is worth mentioning that swelling, mild pain, and light bleeding is common side effects of this procedure, so you don’t have to worry about them.

Here are the things to do after a testicular biopsy:

  • Avoid wearing tight underwear;
  • Take painkillers and antibiotics;
  • Have bed rest for 24 hours;
  • Avoid sexual intercourse for at least a week;
  • Wear an athletic supporter to protect the testicles from pressure and damage;
  • Keep the treated area dry;
  • Avoid taking a shower or washing testes for a couple of days;
  • Avoid taking blood thinners such as aspirin for a week;
  • In case of having pain, take prescribed painkillers or acetaminophen; 


to do after a testicular biopsy

Testicular Biopsy Results

Interpreting the results of the testicular biopsy for IVF is very important in choosing the best treatment. Although testicular biopsy is a simple operation performed only through a small incision in the skin of the scrotum, interpreting its results is essential and difficult. In the fertility center, a pathologist examines the samples. In some cases, the patient is infertile and does not have any germ cells. In this situation, the doctor recommends using a donated embryo for the patient.

In some patients, the germ cells have evolved in several stages, but at a particular stage, this evolution has stopped. This group of people also does not have sperm in their semen, but this group's situation is different from those with no germ cells. Because these people can get better by using particular medicines, and the developmental stage of their germ cells can be completed, or in some cases, their immature sperms can mature in the laboratory. It is worth mentioning that both of these groups suffer from azoospermia. 

Interpretation of Testicular Biopsy’s Result


Interpretation of Testicular Biopsy’s Result

In general, the interpretation of testicular biopsy results is divided into four categories:

  1. Natural: in these cases, testicular problems are not the cause of the lack of sperm.
  2. Stop in sperm evolution: sperm must go through 16 stages to evolve. Sometimes this evolution stops at one point.
  3. Insufficient sperm production: in this case, the complete development of sperm occurs in some parts of the testicle, but these parts are so limited that the sperm does not appear in the semen.
  4. Lack of germ cells: these patients do not have any germ cells in their testicles.


Sometimes the biopsy test shows that the testicle has its normal function, and the lack of sperm in the testicle relates to obstructive azoospermia (OA), which results from a blockage of the male reproductive tract. 

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Concluding Remarks and Recommendations

Regarding the abovementioned points, it seems that today the best way to diagnose a variety of sperm problems (including low count, low quality, low motility, improper shape, etc.) is a testicular biopsy. In addition to its diagnostic use, it provides the basis for using the most appropriate methods to treat male fertility problems.

Therefore, it is urgent to increase the awareness of infertile couples about this operation when they refer to fertility centers and consult a specialist doctor.

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