How to Know if Embryo Transfer has Worked?

Waiting for the embryo to implant in the uterus is the hardest part of fertility treatment for almost all patients. This two-week period can bring about lots of emotions, from the hope that you finally get pregnant to anxiety that the implantation may fail.

The best way to reduce stress is to know more about what symptoms you may experience during this 14-day wait time after embryo transfer and what symptoms can be counted as failed implantation. This article enumerates the positive signs after embryo transfer and answers some of your frequently asked questions.

When to Take a Pregnancy Test After Embryo Transfer?

What Is Embryo Transfer?

Embryo transfer is the last and most important step in all assisted reproductive techniques. The fertility doctor transfers the embryo(s) to the cervix or uterus via a narrow plastic tube. The number of embryos transferred differs according to the quality of embryos, maternal age, and the parent’s expectations.

If the embryos are of low quality or the mother’s age is above 35, the fertility doctor transfers more than one embryo to increase the chance of successful pregnancy. Also, if the parents tend to have twins (triplets), the doctor transfers two or three quality embryos. However, most specialists do not recommend multiple pregnancies as it can risk the mother's and baby's lives. 

after embryo transfer
Read more: What to Do After Embryo Transfer to Increase Success?

Embryo Transfer in IVF and ICSI

The initial procedures of IVF and ICSI are the same, including blood work, physical examinations, and medications that should be taken. In the IVF treatment, the harvested egg and sperm are placed in a laboratory dish where the sperm penetrates the egg and fertilizes it. In this method, the sperm must be healthy enough to fertilize the egg on their own.

In ICSI treatment, conversely, motile and healthy sperm is chosen and directly injected into the center of the egg using a microscope and micropipette (a narrow, tiny needle). This technique is mainly suitable for couples because the male partner’s sperm lacks the necessary parameters (count, motility, concentration, and morphology).

However, the embryo transfer process in both these methods is the same. The embryo is cultured in the laboratory for three to five days. After performing PGT tests, it is transferred to the mother’s (or surrogate’s) uterus using a cannula and ultrasound probe. The first positive signs after IVF or ICSI embryo transfer are cramps, nausea, and vomiting, but you should still take reliable tests -such as blood and urine- to ensure you are pregnant. 

When to Take a Pregnancy Test After Embryo Transfer?

Although taking a pregnancy test soon after embryo transfer is tempting, you must wait 14 days before taking it because the placenta secretes hCG -or pregnancy hormone- 10 to 12 days after embryo transfer. Therefore, it would be better to wait two weeks before taking a beta or home pregnancy test, as these tests can sometimes show false results and make you disappointed and nervous. 

Positive Signs After Embryo Transfer

Before embryo transfer, you must take various fertility drugs containing estrogen and progesterone. These medications may cause symptoms similar to the pregnancy ones; therefore, the best way to determine pregnancy after embryo transfer is by taking a blood test.

However, you are probably pregnant if you have most of the following symptoms:

  • Missed period
  • Bleeding or spotting
  • Breast tenderness and soreness
  • Cramps
  • Fatigue 
  • Nausea and vomiting
  • Bloating 
  • Increase in vaginal discharge
  • Frequent urination

Positive Signs After Embryo Transfer

Missed period

The missed period is the first thing that comes to mind when discussing pregnancy. So, if you have regular menstrual cycles but have missed one, you are probably pregnant. 

Bleeding or spotting

The first and most obvious sign of embryo implantation is light bleeding or spotting seven days after embryo transfer. Seeing drops of blood on your underwear means the embryo has successfully attached to the endometrium.

However, spotting is also a complication of progesterone shots you receive after embryo transfer, so light bleeding might or might not be a pregnancy sign after embryo transfer.

Breast tenderness and soreness

Like in natural pregnancy, you will experience breast tenderness and soreness after embryo transfer because the pregnancy hormones reach their highest level during this time. But, once again, oral and injectable hormones can make your breasts swollen and painful, so it cannot be a definite sign of embryo implantation. 


After embryo transfer, cramps can happen for three reasons; you are about to menstruate, the embryo transfer was successful, and the fetus is attached to the uterus lining, or your pelvis has been hurt during the transfer procedure.

Also, cramping and mild pain in the lower abdomen can be caused by the progesterone you take after the transfer.

Positive Signs After Embryo Transfer; Cramps


Have you ever been so tired during your period that you couldn’t get up from bed? That’s because the progesterone had soared in your blood, making you tired and dizzy. Extreme tiredness is normal during pregnancy as the level of progesterone in the blood is very high. So, fatigue and dizziness can be pregnancy signs after embryo transfer. 

Nausea and vomiting

Morning sickness and vomiting usually begin on the 8th week of pregnancy, not in the first two weeks after embryo transfer. So, nausea and upset stomach are not definite signs of successful implantation. If the cause of your nausea is not anxiety or fertility drugs, you should contact your doctor. 

Positive Signs After Embryo Transfer; Nausea and vomiting


When the number of progesterone increases, it causes various digestive problems, especially bloating and abdomen inflammation. So, you will feel bloated when you are pregnant or take progesterone-containing medications. 

Increase in vaginal discharge

An increase in vaginal discharge in the first seven days after embryo transfer can signify embryo implantation. The discharge during this period is usually white and smelly and probably continues during the first weeks of pregnancy.

Remember that you may also experience abnormal discharges, infection, burning sensation, and itching due to vaginal suppositories, gel, or tablets. So, having more discharges cannot prove that you are pregnant.

Frequent urination

An increase in urination, especially at night, can be an early sign of pregnancy after embryo transfer. The reason for frequent urination during pregnancy is a rise in the levels of hCG and progesterone hormones.

Unfortunately, increased urination can also be caused by infection of the reproductive and urinary tracts. So, if you have painful and frequent urination, fever, severe nausea, vomiting, or blood in your urination, contact your doctor as soon as possible.

No Symptom After Embryo Transfer

Whether using a fresh or frozen embryo, the signs of pregnancy after the embryo transfer are the same. Interestingly, having no symptoms after embryo transfer is completely normal and does not imply that your embryo implantation has failed. In fact, 10 to 15 patients with no symptoms after embryo transfer have had healthy pregnancies resulting in live births.

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Fertility Treatment in Iran

Whether the cause of your infertility is known or not, Iranian fertility specialists can help you have children of your own quickly. In Iran, various assisted reproductive technologies such as IVF, IUI, ICSI, donor sperm/egg, and surrogacy are practiced in standard hospitals and by the hands of proficient, knowledgeable doctors, so you can be assured that your treatment will be 90% successful. Finally and most importantly, the cost of fertility treatments in Iran ranges between 1.000 to 3.000 USD which is incomparable to other countries, especially the Western ones.  

IVF in Iran

FAQs About Positive Signs after Embryo Transfer

How soon after embryo transfer do you feel pregnancy symptoms?

During the first week after embryo transfer, you probably experience pregnancy-like symptoms such as nausea, vomiting, fatigue, and bloating.

How do you know if embryo transfer has worked?

Cramps, white and smelly discharges, and spots are the first positive signs after embryo transfer. However, only a blood test (beta test) can confirm the pregnancy with 100% accuracy. 

How soon after embryo transfer can your test become positive?

You have to wait 10 to 14 days before taking a pregnancy test. If you take a pregnancy test sooner, the results can be false-positive or false-negative. 

Where do you feel embryo implantation is?

Pregnant after embryo transfer, you may have mild pain or cramps in the middle of your abdomen, lower back, lower abdomen, or pelvic area.

How do you know if an embryo has not been implanted?

Heavy bleeding, severe pain in the abdomen, and brown discharges can imply that embryo implantation has failed. However, you cannot be sure your embryo has not been implanted until you take a pregnancy test or undergo an ultrasonic examination. 

Do frozen embryos take longer to implant?

Generally, there is no difference between fresh and frozen embryos regarding the speed of implantation. If the embryo has high quality, is genetically normal, and the endometrium is receptive, the fresh/frozen embryo can grow at normal speed and result in a healthy pregnancy.


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